Cell viability was assessed at different times with MTT (3-(4,5-Dimethythiazol–2-yl)-(2,5-diphenyltetrazolium) bromide) assay. After treatments, MTT was added (final concentration 0.5 mg/mL) and cells were incubated for 3 h. Formazan crystals were dissolved and absorbance measured at 570 nm in microplate reader FL600 (BioTek, Winooski, VT, USA). Each experimental group had three duplicate wells.

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