Colony of Z. cucurbitae was reared for many generations in the insect rearing room at 25 ± 1°C and 75% relative humidity, with a 14:10 h (light: dark) photoperiod at Hainan University, Haikou, China. Larvae were fed with artificial food as described previously (Liu et al., 2020). Fruit flies were reared on a ratio of 3:1 of sugar and yeast for around 10–12 generations in 45 cm × 45 cm × 50 cm cages before the experiment to eradicate local environmental impact.

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