Three spikelets from each plant (three plants for each material) were collected for pollen fertility experiments. Pollen grains at the mature stage were stained with 1% iodine-potassium iodide solution (I2-KI) solution and photographed using a Nikon DS-Ri2 microscope. Five fields of each sample with at least 50 pollen grains per visual field were counted. Abnormally sized and unstained pollens were counted as “abnormal”. The pollen number were counted using ImageJ software.

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