2.4. Reverse-Transcription Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR) Assays

Lyophilized mycelia were used for isolation of total RNA as described previously by Chomczynski [36]. RT-qPCR assays were carried out as detailed in Kovács et al. [37], with the primer pairs listed in Table S1 and using the actA gene as reference. Relative transcription was characterized with either ΔCP or ΔΔCP. ΔCP = CPr − CPt where r, t, and CP stand for the reference gene, target gene, and the crossing point value detected in the RT-qPCR assays, respectively. ΔΔCPtreatment = ΔCPtreated culture − ΔCPuntreated culture and ΔΔCPmutation = ΔCPmutant − ΔCPreference strain.

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