2.2. Recording Growth, Measuring GSH and GSSG Contents, 2′,7′-Dichlorofluorescein-Assay (DCF-Test)

Mycelia from 10 mL fermentation broth were filtered and dried at room temperature. The increase in the dry cell mass (DCM) values between 16 h and 26 h were calculated and used to characterize the growth of CdCl2-treated and untreated cultures.

Mycelia samples taken at 18.5 h and treated with 5′-sulfosalicylic acid [27,28] were used for the determination of (reduced) glutathione (GSH) and oxidized glutathione (GSSG) content with the DTNB-glutathione reductase assay [29] as well as for DCF-test to record changes in the redox homeostasis of the cultures [30].

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