To verify viral expression, frozen brain sections were prepared. Mice were anesthetized with pentobarbital (80 mg/Kg bodyweight, i.p.) and transcardially perfused with 20 mL of 0.9% saline, followed by 20 mL of 4% paraformaldehyde (PFA) in PB (0.1 mol/L, pH 7.4). After perfusion, each brain was harvested, postfixed for 24 h in PFA at 4°C, and then cryoprotected with 30% sucrose in PBS until the tissue sank. The brain was then embedded in OCT compound and sectioned at 30 μm on an upright Leica cryostat. Tissue sections were mounted on slides and imaged by a confocal scanning microscope (Zeiss, LSM710).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.