To verify viral expression, frozen brain sections were prepared. Mice were anesthetized with pentobarbital (80 mg/Kg bodyweight, i.p.) and transcardially perfused with 20 mL of 0.9% saline, followed by 20 mL of 4% paraformaldehyde (PFA) in PB (0.1 mol/L, pH 7.4). After perfusion, each brain was harvested, postfixed for 24 h in PFA at 4°C, and then cryoprotected with 30% sucrose in PBS until the tissue sank. The brain was then embedded in OCT compound and sectioned at 30 μm on an upright Leica cryostat. Tissue sections were mounted on slides and imaged by a confocal scanning microscope (Zeiss, LSM710).

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