Argininosuccinate synthetase 1 (ASS1) staining and reporting followed the previous protocol.5,20 Immune phenotyping was performed for CD3 (Cat#A0452, Dako/Agilent, Santa Clara, CA, USA) and PD-L1 (Cat#13684S, Cell Signaling, Danvers, MA, USA), staining were conducted as described previously.21

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