The virus injection procedure was similar to that described in our previous studies [6, 28]. Briefly, in Thy1-cre mice, the mitral cell layer of the OB was injected with AAV-DIO-GCaMP6s virus (PT-0071, AAV2/9, 5.03×e+12 vg/mL, BrainVTA, Wuhan, China). All injections were made with a glass pipette and the injection volume and velocity were controlled by a microsyringe pump (Quintessential Injector; Stoelting Co.). Virus solutions (300 nL) were injected at 30 nL/min into the mitral cell layer (4.2 mm anterior, 1.0 mm lateral, and 0.8–1.1 mm ventral to bregma). The glass pipette was left in place for an additional 10 min before being slowly withdrawn. After the viral injection, the scalp was sutured. Mice were individually housed for at least three weeks after surgery for recovery and to allow time for the expression of GCaMP6s.

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