Eleven male Thy1-cre [FVB/N-Tg(Thy1-cre)1V1n/J] mice aged 8–16 weeks were used in this study. The mice were bred in the animal facilities of Xuzhou Medical University and housed in a vivarium under a 12 h/12 h light/dark cycle, with lights on at 08:00. Experiments were performed during the light cycle. After surgery, the mice were housed individually for at least 10 days before further experiments to allow recovery. Food and water were available ad libitum. All experimental procedures were performed in accordance with protocols submitted to and approved by the Xuzhou Medical University Institutional Animal Care and Use Committee.

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