All subjects were recruited at National Cheng Kung University Hospital. Eligible subjects were over 18 years of age, with a confirmed advanced solid tumor that failed prior systemic therapy, measurable disease, archival tumor tissue, ECOG performance status of 0 or 1, adequate hematologic, hepatic, and renal function and an expected survival of at least 3 months. No anti-cancer therapy could be received in the 2 weeks prior to study entry. Additionally, patients with uncontrolled intercurrent illness, expected noncompliance, history of another primary cancer unless unlikely to affect outcome, prior ADI-PEG 20 therapy, prior immunotherapy, and known allergy to ADI-PEG 20 were ineligible.

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