The LC–MS analysis was performed with the use of an Agilent 6520 series high-resolution Q-TOF system and a UHPLC 1290 series system (Agilent Technologies, Santa Clara, CA, USA) with a Kinetex C18 (2.1 × 50 mm, dp = 1.7 μm) column and a C18 precolumn guard (Phenomenex, Torrance, USA). In order to perform both qualitative and quantitative analysis of the studied processes, the MS detector was operated in the positive mode in the extended dynamic range (2 GHz). MassHunter workstation software version B.04.00 was used for the control of the system, data acquisition, qualitative and quantitative analysis. To ensure accuracy in mass measurements, a reference mass correction was applied and masses 121.050873 and 922.009798 were used as lock masses. MS detection based on the extracted ion current chromatograms (EIC) was applied for the quantitative analysis of dapoxetine and its metabolites; next, the automatic MS/MS mode (using the abundance algorithm) was used to register their fragmentation spectra. All the chromatographic and spectrometric parameters are described in Table S1.

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