Six-week-old female BALB/c mice were injected with 177Lu-labeled NMGs (200 pmol, 100 µL, 2 µM in PBS, 20 MBq, 20 µg/kg) via the tail vein. The animals were sacrificed via CO2 asphyxiation, and samples of blood and urine (100 µL) were collected at 10 min post injection (p.i.). For blood samples, proteins were precipitated immediately by addition of CH3CN (100 µL), and the samples were centrifuged (3 min, 20238 rcf, rt). The supernatant was diluted with water (1:1), and metabolites were analyzed via γ-HPLC. Urine samples were centrifuged, diluted with water (1:5), and metabolites were analyzed by γ-HPLC. n = 2–3 animals per group.

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