The ws and bs colonies of the An. albimanus Tapachula strain were reared at insectary conditions of 28 ± 1°C, humidity at 80% and 12 hours photoperiod. Larval stages were fed with cat food (chicken, cereals, and milk mix) for 2–12 months kitten (Whiskas) [91]. The ws and bs phenotypes have been reared independently for more than sixty generations resulting in stable colonies (S1 Fig). Adult mosquitoes were fed with 10% sugar solution using soaked cotton pads. For Anopheles mosquitoes, the sugar solution was supplied with 4-Aminobenzoic acid (PABA) 0.05% and antibiotic-antimicotic 1X (GIBCO). The An. albimanus colonies and the Ae. aegypti Rockefeller strain were reared at the insectary of the National Institute of Public Health (INSP) in Cuernavaca, Morelos, México. The Ae. aegypti New Orleans strain was reared at the insectary of the Collaborative Unit for Entomological Bioassays (UCBE) of the Autonomous University of Yucatán (UADY) in Mérida, Yucatán, México. Larval stages were fed with a meat flour and yeast (8:2) solution. Adult mosquitoes were fed with 10% sugar solution using a felt strip device. The metal ion content of the two diets was determined (S2 Table).

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