The effect of LGEO on S. Heidelberg motility was examined using a motility assay (Niu and Gilbert, 2004). The semi-solid agar was made using 20 mL of Luria-Bertani broth (LB; catalog no. C6001, CulGenex, Hardy Diagnostics, Santa Maria, CA), 0.3% (w/v) agarose (catalog no. C5001, Criterion, Hardy Diagnostics, Santa Maria, CA) and poured into Petri dishes (100 by 15mm; VWR). Treatment groups were supplemented with either 0.0075%, 0.015%, or 0.15% (vol/vol) LGEO into the agar mix before pouring onto plates. Then, 10 µL of either S. Heidelberg isolates (∼5.0 log10 CFU) were inoculated at the center of the plates and incubated for 8 h at 37°C before the zone of motility diameter was measured. Appropriate controls were kept alongside treatments, and at least 8 replicates were included for each strain.

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