The results of the former assay were validated using a macro-broth dilution assay. Separate macro-broth dilution assays were conducted for each strain. Briefly, 10 mL of TSB was inoculated with ∼5.0 log10 CFU/mL of either isolate, supplemented with either 0.0075%, 0.15%, 0.5%, or 1% (vol/vol) of LGEO, and incubated at 37°C. Negative controls (without Salmonella or LGEO) and positive controls (with Salmonella without LGEO) were also included. S. Heidelberg was enumerated by serial dilution in PBS and surface plating 0.1 mL on XLD before incubation, and after 8 and 24 h of incubation. Duplicate samples were kept for all treatments, and the experiment was repeated 3 times.

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