Animal experiments were performed with protocols approved by the Institutional Animal Care Committee at Beijing Institute of Biotechnology. Ten million A549 cells stably expressing RHOV shRNA or control shRNA were injected subcutaneously in the right flank of each nude mice. Tumor size was examined at the indicated times. The mice were euthanized at the indicated time. Excised tumors were perserved in liquid nitrogen.

For tumor metastasis analysis, 1 × 107 A549 cells stably expressing RHOV shRNA or control shRNA were injected into the tail vein of each nude mice. After euthanasia at the indicated time, all lung metastatic foci were investigated histologically.

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