Identification of metastasis driver genes (MDGs) and construction of Protein-protein interaction (PPI) network
This protocol is extracted from research article:
RHOV promotes lung adenocarcinoma cell growth and metastasis through JNK/c-Jun pathway
Int J Biol Sci, Jun 22, 2021; DOI: 10.7150/ijbs.59939

To investigate differentially expressed genes (DEGs) between LUAD tissues with non-metastatic predisposition and LUAD tissues with metastatic predisposition in TCGA-LUAD dataset, we used the limma R package 12. DEGs were set as follows: False Discovery Rate (FDR) <0.05 and |log2 fold change (FC)|>1. Among DEGs, a PPI network of MDGs was built by the Search Tool for the Retrieval of Interacting Genes (STRING, www.string-db.org/), and was organized and visualized by Cytoscape software.

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