Isolated IgG samples were dried in a vacuum centrifuge. After drying, IgG was denatured with the addition of 30 μl of 1.33% SDS (w/v) (Invitrogen, USA) and by incubation at 65 °C for 10 min. Plasma samples (10 μl) were denatured with the addition of 20 μl of 2% SDS (w/v) (Invitrogen, USA) and by incubation at 65 °C for 10 min. From this point on, the procedure was identical for both IgG and plasma samples. After denaturation, 10 μl of 4% Igepal-CA630 (v/v) (Sigma Aldrich, USA) was added to the samples, and the mixture was shaken 15 min on a plate shaker (GFL, Germany). N-glycans were released with the addition of 1.2 U of PNGase F (Promega, USA) and overnight incubation at 37 °C.

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