We have analysed a total of 6032 plasma samples from 2146 participants of the TwinsUK study, collected at multiple timepoints over a 20 year-period, as a replication cohort [28]. These included 1865 individuals sampled at three timepoints, 156 individuals sampled at two timepoints and 125 individuals sampled only once. Following the plasma N-glycome analysis, glycan data underwent quality control (see Statistical analysis section), which decreased the dataset to 5889 samples (measurements). Out of these 5889 measurements, we have proceeded with statistical analysis on a subset of 3742 samples (measurements) that had information on BMI available. Description of the TwinsUK cohort is provided in Table Table22.

Demographic characteristics of the TwinsUK cohort.

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