Kjeldahl catalyst tablets were from VWR International (Dublin, Ireland). Low nitrogen sodium hydroxide (40% (w/v)) and sulphuric acid were from TE Laboratories Ltd. (Carlow, Ireland) and Lennox Laboratory Supplies Ltd. (Dublin, Ireland), respectively. The enzyme preparations, BC pepsin and Corolase® PP, were kindly provided by Biocatalysts Ltd. (Cardiff, Wales, UK) and AB Enzymes (Darmstadt, Germany), respectively. Trinitrobenzenesulphonic acid (TNBS) reagent was from Medical Supply Co Ltd. (Dublin, Ireland). Diprotin-A (IPI: Ile-Pro-Ile) and H-Gly-Pro-7-amino-4-methyl coumarin (AMC) were purchased from Bachem Feinchemikalien (Bubendorf, Switzerland). Hank’s buffered saline solution (HBSS 10×), RPMI-1640 culture media, trypsin/EDTA (10×), penicillin-streptomycin (0.1 g/L) and fetal bovine serum (FBS) were bought from Gibco Life Technologies Ltd. (Paisley, Strathclyde, UK). Rat insulin standard and radio-labelled sodium iodide (Na 125I, IMS 100 mCi/mL) were obtained from Novo Industria (Copenhagen, Denmark) and Perkin Elmer (Buckinghamshire, UK), respectively. The other reagents, as well as DPP-IV (human recombinant, expressed in Sf9 cells; ≥4500 units/μg protein), were purchased from Sigma Chemical Company Ltd. (Wicklow, Ireland).

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