Quantification of fluorescent immunostaining results was done with Fiji version of ImageJ (Fiji, RRID:SCR_002285). Pain behaviors were analyzed using two-way ANOVA with multi comparisons to test for main effects between groups followed by post-hoc testing for significant differences. Two-group analysis utilized the Student’s t-test. Three to four group analyses utilized a one-way ANOVA design. Data are expressed as mean ± SEM. All statistical analyses were performed using GraphPad Prism 8 software (GraphPad Prism 8, RRID:SCR_002798). Level of significance is indicated with *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.