Quantification of fluorescent immunostaining results was done with Fiji version of ImageJ (Fiji, RRID:SCR_002285). Pain behaviors were analyzed using two-way ANOVA with multi comparisons to test for main effects between groups followed by post-hoc testing for significant differences. Two-group analysis utilized the Student’s t-test. Three to four group analyses utilized a one-way ANOVA design. Data are expressed as mean ± SEM. All statistical analyses were performed using GraphPad Prism 8 software (GraphPad Prism 8, RRID:SCR_002798). Level of significance is indicated with *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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