Statistical analyses were conducted using Microsoft Excel and Prism (GraphPad). Statistical significance was determined either by two-tailed Student's t-test, one-way ANOVA using the Bonferroni comparisons test with a 95% confidence interval or nested ANOVA, as indicated in the figure legends. Significance was calculated, where * indicates p<0.05, ** indicates p<0.01, *** indicates p<0.001, and **** indicates p<0.0001.

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