WT HeLa and cavin3 KO cells were seeded at low density (500 cells/well) in six-well plates, left untreated or treated with 5 nM concentrations of PARP (AZD2461), and were allowed to form colonies for 6 days. Colonies were fixed and stained with 0.5% crystal violet/20% ethanol and counted. Results were normalized to plating efficiencies where the

Plating efficiency (PE) = no. of colonies formed/no. cells seeded × 100% and

Survival fraction (SF) = no. of colonies formed after PARP treatment/no. cells seeded × PE × 100%

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