Crushing dorsal skin samples (100 mg) in 900 μL radioimmunoprecipitation assay lysis buffer (Beyotime Biotechnology, Shanghai, China) containing 2% (v/v) protease inhibitor mixture (Beyotime Biotechnology) and 2% (v/v) phosphatase inhibitor mixture (Beyotime Biotechnology) with the grinder (Scientz48, Scientz Biotechnology, Ningbo, Zhejiang, China) at 60 Hz for 30 s, five times. According to the manufacturer’s instructions, IL-22, IL-23, and IL-17 levels were measured using enzyme-linked immunosorbent assay (ELISA) kits (Enzyme-linked Biotechnology, Shanghai, China), and total protein levels were measured using an enhanced bicinchoninic acid protein assay kit (Beyotime Biotechnology).

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