AGC cells grown on cover-slips inside a petri dish were transfected with RORβ/pReceiver plasmid and control and were incubated at 37°C for 2 days using Lipofectamine® 3000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.). The cells were washed once with PBS. Then, the cells were incubated with 3–4% formaldehyde in PBS at room temperature for 30 min. The staining solution was aspirated carefully and the fixed cells were washed 2–3 times in PBS. Conjugate working solution 1X Phalloidin-iFluor™ 488 (AAT Bioquest, Inc.) was added to the fixed cells. Subsequently, the cells were incubated at room temperature for 60 min. DAPI staining solution (BIOSS) was then added and the cells were incubated at room temperature for 5 min. The cells were rinsed gently 2–3 times with PBS to remove excess phalloidin conjugate. Mounting medium was added and sealed. The cells were observed at a magnification of ×400 under a fluorescence microscope (Olympus Corporation) with an FITC filter set.

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