Sequence analysis and neighbor-joining tree

The promoter sequence of BjMYB113 alleles was analyzed using PlantCARE (

Sequence alignments were conducted using Muscle program and manually adjusted in GeneDoc ( The neighbor-joining tree was constructed using MEGA 7.0 [54] and bootstrap values were calculated using 1,000 times.

The gene sequences (BjMYB113a, -b, and -c) supporting the results of this study are available in the NCBI GenBank ( under accession number MW166171- MW166173 of BankIt2394807. The primers used in gene sequences analysis were shown in Table S3.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.