Sequence analysis and neighbor-joining tree

The promoter sequence of BjMYB113 alleles was analyzed using PlantCARE (http://bioinformatics.psb.ugent.be/webtools/plantcare/html/).

Sequence alignments were conducted using Muscle program and manually adjusted in GeneDoc (http://www.nrbsc.org/gfx/genedoc/). The neighbor-joining tree was constructed using MEGA 7.0 [54] and bootstrap values were calculated using 1,000 times.

The gene sequences (BjMYB113a, -b, and -c) supporting the results of this study are available in the NCBI GenBank (https://www.ncbi.nlm.nih.gov/genbank/) under accession number MW166171- MW166173 of BankIt2394807. The primers used in gene sequences analysis were shown in Table S3.

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