MKN45 and AGS cells were plated into 96-well plates and incubated overnight. Approximately 1×104 cells in each well were serum-starved overnight, and then the head of the pipette tip was used to produce a scratch in the cell monolayer. The cells were then washed with PBS 3 times to remove the unattached cells and subsequently incubated in fresh culture medium without FBS for indicated time-points at 37°C. Images of the wound were captured at 0, 12 and 24 h using a light microscope (magnification, ×20). The percentage of migration area was calculated using ImageJ software (1.52v; National Institutes of Health).

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