Plasmid construction and plant transformation

The full-length BjMYB113 cDNA was cloned into the pRI101-GFP vector with CaMV 35 S promoter to construct the overexpression vector. The full-length BjMYB113 gene sequence (including native promoter and 3’region sequence) was amplified and cloned into the pRI101-GFP to construct the complementary vector. Approximately 3 kb promoter region of three BjMYB113 alleles were amplified and recombined into the pCAMBIA1301-GUS vector for checking GUS activity. All vectors were constructed using homologous recombination. Positive plasmids were verified by sequencing and then transformed into Agrobacterium tumefaciens GV3101 using thermal stimulation method. The vectors were transformed into Arabidopsis using floral-dip method [52]. All primers used for vector construction were shown in Table S3.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.