All animal experiments were approved by the Institutional Animal Care and Use Committee of Zhejiang University (Hangzhou, China) and complied with the Animal Welfare Act (20). BALB/c nude mice (30 females; 4 weeks old; body mass 17–20 g) were purchased from Shanghai Laboratory Animal Center. The mice were bred at the Laboratory Animal Research Center at Sir Run Run Shaw Hospital in a barrier environment, specific pathogen-free (SPF) grade, with humidity approximately 30 to 50% at 22°C. In addition, water and food was provided ad libitum. A total of 0.2 ml RORβ-overexpression GC cells and control cells (at the density of 1×105, 5×105 and 1×106) were subcutaneously inoculated into the backs of nude mice. There were five mice in each group. Tumor growth was observed weekly, and rats were sacrificed by cervical spinal cord transection after 4 weeks, and the tumor body was measured and analyzed. The tumor volumes were assessed according to the diameters of the tumors.

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