The endothelin-1 (ET-1) (#CSB-E08322h, CUSABIO) and eNOS (#CSB-E07007h, CUSABIO) level from the HUVEC culture medium in each sample were measured using a commercial enzyme immunoassay technique-based kit. The plate was read for absorbance at 450 nm on a microplate spectrophotometer (GENios Plus, Tecan), and samples were compared to the obtained standard curve.

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