RORβ-overexpression GC cells and control cells were trypsinized, and 1×106/ml cells were collected by centrifugation (300 × g, 5 min at 4°C). Cells were washed with PBS twice and incubated in 500 µl binding buffer, 5 µl Annexin V-EGFP and 10 µl propidium iodide at room temperature in the dark for 15 min using Annexin V-FITC/PI Apoptosis Detection Kit (Beyotime Institute of Biotechnology) according to the manufacturer's instructions. Apoptotic cells were visualized and quantified using FACSAria (BD Biosciences) with FlowJo software (version 7.6.1; Tree Star, Inc.).

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