RORβ-overexpression and RORβ-knockdown GC cells and control cells were digested and cultured in 24-well plates at a density of 200 cells/well in serum-free medium incubated at 37°C with 5% CO2 for 7 days. The medium was replaced every 3–4 days. Following the incubation, the spheres were counted manually using a light microscope with a magnification of ×20.

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