The relative quantities of flavonoid metabolites in Brassica juncea leaf samples were analyzed with a liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) system by MetWare (Wuhan, China). The liquid chromatography–electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) system was used for the relative quantification of anthocyanin metabolites in Brassica juncea leaves samples. The protocols were described detailedly in Methods S1.

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