Plant materials and genetic segregating populations

Purple mustard cultivar pl102 and green cultivars rt104 and gre101 were chosen to study the genetics of purple color leaves. These three mustard cultivars are brown mustard (Brassica juncea). Purple cultivar pl102 was crossed with green cultivars rt104 and gre101, respectively, to develop two F2 segregating populations. These F2 segregating populations along with its parents were acquired from professor Hanhui Kuang’s lab (College of Horticulture and Forestry Sciences, Huazhong Agricultural University) in this study. Parental seed lines were originally acquired at National Center for Vegetable Improvement (Central China). Seeds were germinated and seedings grew in a greenhouse under 16/8 h photoperiod at 25 °C ± 2 °C. All plants were planted to the field on campus of Huazhong Agriculture University, Wuhan, China.

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