Protein concentrations were determined using the G250 assay (Keygen). Proteins were separated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) by electrophoresis, then transferred to polyvinylidene difluoride (PVDF) membranes. A PTPRF monoclonal antibody (rabbit; 1:2,000; Abcam, UK) were incubated with the PVDF membranes overnight, followed by incubation with an anti-rabbit secondary antibody (1:10,000; Proteintech, USA). The immune complexes were detected using the Two-color Infrared Laser Imaging System (Odyssey).

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