Total genomic DNA was extracted using an Ezup Column Genomic DNA Purification Kit (Sangon Biotech, Shanghai, China), according to the manufacturer’s protocol. The quality and quantity of the extracted DNA were assessed by the ratios of 260 nm/280 nm and 260 nm/230 nm with a NanoDrop spectrophotometer (A360, AOE, Shanghai, China). The V3–V4 region of the bacterial 16S rRNA gene and ITS1 region of the fungi were amplified using TransStart Fastpfu DNA Polymerase (TransGen, Beijing, China). Then PCR products were purified using the SanPerp Column PCR Product Purification Kit (Sangon Biotech, Shanghai, China). The 16S rRNA and ITS1 gene amplicons were sequenced using Illumina HiSeq deep sequencing (Majorbio, Shanghai, China).

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