RNA was isolated from tissues and cells using the Trizol Reagent (TAKARA, Japan). The total RNA was reverse transcribed into cDNA using the Master Mix kit (TAKARA, Japan). Then, the Mir-X miRNA First-Strand Synthesis Kit (TAKARA, Japan) was used in the transcription of miRNA. Quantitative Real-time PCR (qPCR) was carried out with SYBR-Green Master Kit (TAKARA, Japan). The expression levels of target genes were normalized to glyceraldehyde 3-phosphate dehydrogenase (GADPH). The forward primer sequence of LncRNA TCONS_00004099 was 5'-CCTAACAGGTGCTGTGGCA-3', while the reverse sequence was 5'-TCATCGTTGCAGAACAATGGC -3'. The forward primer sequence of GAPDH was 5'-GCTCTCTGCTCCTCCTGTTC-3', while the reverse primer sequence was 5'-AAATGAGCCCCAGCCTTCTC-3'.

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