The rats fasted for 12 h were given a bolus of glucose (2 g/kg). Blood glucose was measured in samples obtained from the tail vein at 0, 30, 60, 90 and 120 min after glucose administration using a glucometer.

We monitored blood glucose level on an empty stomach at 9:00 am, after 8–12 h of fasting every 2 weeks during the experiment.

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