Oligonucleotide synthesis and transfection, lentivirus vector transfection
This protocol is extracted from research article:
LncRNA TCONS_00004099-derived microRNA regulates oncogenesis through PTPRF in gliomas
Ann Transl Med, Jun 1, 2021; DOI: 10.21037/atm-21-2442

Cells were transfected with lentivirus vector pZHLV-CMV-zsGreen1-puro packaged with the lncRNA TCONS_00004099 overexpression sequence or short hairpin RNA (shRNA). The synthetic inhibitor or mimics of miRNA derived from lncRNA TCONS_00004099, and small interfering RNA (siRNA) were transfected into cell lines using Lipofectamine 3000 (Thermo, MA, USA). All the oligonucleotides and scramble sequences were designed and provided by GenePharma (Shanghai, China). Lentivirus vector pZHLV-CMV-zsGreen1-puro packaged by DongBio.Co.Ltd (Shenzhen, China). The sequences of lncRNA TCONS_00004099 and the related miRNA were demonstrated in Supplementary file.

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