ILSDA methods were performed as previously described90. All serum samples were diluted at 1:40. Final data were reported as percent inhibition. This value was calculated by relating the level of Plasmodium falciparum 18S rRNA detection in the experimental sample to the calculated average of 18S rRNA detection in a condition, where sporozoites were incubated with human hepatocytes in the absence of mouse serum. Navy falciparum sporozoite 1 (NFS1), an IgG1 monoclonal antibody to the P. falciparum CSP major repeat served as the positive control for ILSDAs and was generated by the Naval Medical Research Center for this purpose.

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