All compounds were purchased from Sigma-Aldrich (MO, United States). To determine the minimum inhibitory concentrations (MIC), the broth-dilution method was applied, as standardized by the Clinical Laboratory Standards Institute (CLSI 2012), with minor adjustments. Briefly, bacteria that had been grown for 16 h, 28°C in LB were normalized to 1 × 108 colony forming units (CFU) mL–1 with fresh liquid LB. Then, 190 μL of LB containing twofold serial dilutions of each of the tested compounds were inoculated with 10 μL of bacterial suspension containing 1 × 106 CFU in 96-well microtiter plates for 24 h, 28°C with continuous shaking at 150 rpm. The MIC was recorded as the lowest concentration of compound that was able to inhibit the visible growth of bacteria.

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