We downloaded the gene expression microarray containing HCC tissue and normal control tissue or paracancerous normal tissue from The Cancer Genome Atlas (TCGA, https://cancergenome.nih.gov) and the Gene Expression Omnibus (GEO; https://www.ncbi.nlm.nih.gov/geo), and obtained RNA-sequencing (RNA-seq) transcriptome data, tumor mutation simple-nucleotide variant (SNV) data, and related clinical data of primary HCC patients. Using VarScan software, we counted the base mutation number, calculated the mutation number per trillion base of each sample, obtained the resultant mutation load, and further determined the relationship between mutation load and overall survival (OS).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.