The authenticated colorectal cancer cells (DLD1) were obtained from LGC Standards (Teddington, Middlesex, United Kingdom), grown in adhesion, and propagated in a RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS), 1% L-glutamine 200 mM, and 1% penicillin/streptomycin solution 100 U/mL (all purchased by Euroclone, Pero, Italy). Cells were maintained at 37 °C in a humified atmosphere containing 5% CO2.

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