The authenticated colorectal cancer cells (DLD1) were obtained from LGC Standards (Teddington, Middlesex, United Kingdom), grown in adhesion, and propagated in a RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS), 1% L-glutamine 200 mM, and 1% penicillin/streptomycin solution 100 U/mL (all purchased by Euroclone, Pero, Italy). Cells were maintained at 37 °C in a humified atmosphere containing 5% CO2.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.