The samples were prepared by drop casting 1 µL of a BGNP suspension on a formvar-coated copper grid cleaned with oxygen plasma (200 mesh, Ted Pella, Redding, CA, USA) and were left to dry in air for 2 h. A JEOL JEM 1400 microscope (JEOL, Tokyo, Japan) operating at 120 kV was employed for imaging. The images were analyzed using ImageJ to estimate the mean BGNP diameter (average of the longest tip-to-tip distance).

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