STP0404 was incubated with human, monkey, dog, rat and mouse plasma. These incubations were carried out at a test concentration of 5 μM with an incubation period of 60 mins. Samples of human, monkey, dog, rat and mouse were taken at 0, 15, 30, 45, 60 mins. And stop the reaction by taking 50 μL aliquots to 400 μL acetonitrile with internal standard. Propantheline was used as positive control for human, monkey and mouse plasma and mevinolin as the positive control for dog and rat plasma. The remaining percentage was tested. This test was conducted by a fee to service through Pharmaron (Beijing, China).

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