A liver microsome (LM) stability assay was six-time points of incubation at 0, 10, 20, 30 and 60 min with a 1 μL STP0404 initial concentration. All plates were shaken and centrifuged at 3200 x g for 20 mins. Then 100 μL of supernatant was taken from each well and diluted with 300 μL pure water before analyzed by LC/MS/MS. Animal and human liver microsomes were purchased from Wuxi AppTec, Xenotech or Corning and stored in a freezer (lower than -60°C) before use (Wuxi AppTec, China).

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