The heart, liver, spleen, lung and kidney of the mouse were taken out and fixed in 10% neutral formalin for 48 h. After dehydration, they were embedded in paraffin according to standard methods to prepare tissue sections with a thickness of 5 μm. Hematoxylin and eosin (H&E) staining: H&E staining was performed according to standard methods [26], and histopathological changes were observed using Nikon TE2000 fluorescence microscope (Nikon, Japan).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.