2.12. Human Lung Cancer Xenograft Mouse Model
This protocol is extracted from research article:
Inhibitory Effect of pH-Responsive Nanogel Encapsulating Ginsenoside CK against Lung Cancer
Polymers (Basel), May 28, 2021; DOI: 10.3390/polym13111784

In order to explore the anti-tumor effect of CK-Ngs in vivo, a nude mouse model of human lung cancer A549 was established. 4-week-old female BALB/Chinese nude mice (16 ± 2 g) were purchased from Hunan SJA Laboratory Animal Co., Ltd., Changsha, China. After the mice were acclimatized under sterile conditions for one week, A549 cells (1 × 107 cells per mouse) were inoculated into the left forelimb fossa of the mice. When the tumor volume reached about 200 mm3, the mice were randomly divided into three groups (n = 5). Through intraperitoneal injection, the control, free CK and CK-Ngs (20 mg/kg CK equivalents). During the experiment, the body weight and tumor size of the mice were measured every three days, and the tumor volume was calculated as follows: tumor volume (mm3) = (length × width 2)/2. After 21 days of treatment, the mice were sacrificed humanely by cervical dislocation, the tumors and major organs were obtained by dissection, weighed and imaged. All experiments were conducted in accordance with the People’s Republic of China Animal Ethics Regulations and People’s Republic of China Animal Ethics Guidelines and were approved by the Animal Ethics Committee of Northwest University (NWU-AWC-20201018).

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