2.7. In Vitro pH-Responsive Drug Release
This protocol is extracted from research article:
Inhibitory Effect of pH-Responsive Nanogel Encapsulating Ginsenoside CK against Lung Cancer
Polymers (Basel), May 28, 2021; DOI: 10.3390/polym13111784

The in vitro drug release of CK from CMC-β-CD Ngs was assessed by membrane dialysis method [24]. The CK was released in PBS (pH 7.4) and acetate buffer (pH 5.8), containing 1% Tween 80 for more than 120 h. In Brief, 2 mL CK-Ngs suspensions (5 mg/mL) were transferred into dialysis bags (MWCO 3500 Da) and then immersed in 50 mL tubes containing 40 mL release medium. The tubes were placed in an orbital water bath shaker at 37.0 ± 0.5 °C and shaked it at a speed of 120 rpm. Then, 5 mL of release medium was taken out and replaced it with an equal amount of fresh release medium at the time points of 0, 3, 6, 12, 24, 36, 48, 60, 72, 96, 120 and 144 h respectively. The cumulative release percentage of CK from the dialysis bags was determined by HPLC.

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