2.5. Drug-Loading and Encapsulation Efficiency
This protocol is extracted from research article:
Inhibitory Effect of pH-Responsive Nanogel Encapsulating Ginsenoside CK against Lung Cancer
Polymers (Basel), May 28, 2021; DOI: 10.3390/polym13111784

The concentration of CK in the CMC-β-CD Ngs was determined using high performance liquid chromatography (HPLC; Agilent 1260 Infinity) [23]. The lyophilized samples were accurately weighed and dissolved in acetonitrile, sonicated for 30 min, filtered with a 0.45 μm membrane filter, and analysed by HPLC. The Eclipse Plus C18 column (4.60 mm × 250 mm, 5 µm) (Agilent, Santa Clara, CA, USA) was used for separation, and gradient elution chromatography was used with mobile phase of water (A) and acetonitrile (B). The flow rate was 1.5 mL/min, the injection volume was 20 µL and the column temperature was set at 35 °C. The ultraviolet wavelength was set to 203 nm. The linear regression equation of the calibration curve for CK in the test range of 5–100 µg/mL was y = 4.4311 x + 0.4386, R2 = 0.9992 (y: the peak area, x: CK concentration, µg/mL). Drug loading (DL) and entrapment efficiency (EE) of CK-Ngs were calculated by Equations (2) and (3).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.